Contract Research Services

Aumgene offers a wide range of contract research services to Biotech, Life sciences and Pharmaceutical companies in India and abroad.

A partial list of contract research services that we offer:

1. Microbial strain development & optimization for production of industrial and Speciality enzymes, Neutraceuticals (Probiotics and Prebiotics), Pharmaceutical intermediates, Biotransformation, Biochemicals, etc.
2. Molecular cloning & Protein purification for expression of recombinant proteins for therapeutic and non-therapeutic applications.

• Gene cloning
  Gene Synthesis:  Target gene will be isolated and codon optimization of the gene to suit the one in the expression system will be done to increase the expression level.
  Gene insertion:  Synthesized gene will be ligated in a suitable expression vector. Sequencing and restriction enzyme analysis will be done to verify the identity of the target gene.

• Fermentation
  Selection of the highest producer by screening the positive clones will be done. Lab fermenter level production will be done.
  Depending on product and customer requirement pilot scale fermentation batches and production scale up services can also be offered. We presently have fermenters of following capacities (working volume);
   
• 7 L
• 20 L
• 50 L
• 100 L
• 300 L
• 500 L
• 3000 L

• Downstream processing & Purification
  Fermentation cell mass will be processed to carry out isolation and purification of the target proteins.
  For industrial proteins we can use Microfiltration or Ultrafiltration to get the desired protein concentration.
  For therapeutic proteins or for high protein purity requirements, purification will be done through column chromatography using wide range of columns.
  Refolding will be done using various techniques to achieve the correct conformation. [Optional]
   

• Protein Characterization
  Physicochemical characterization of purified protein will be done by following techniques.
• SDS-PAGE [Coomassie stained and Silver stained] 
• Western Blot analysis [customer supplied antibodies needed].
• Isoelectric Focusing 
• Molecular Weight determination [SDS-PAGE and Mass Spectra]
• Percentile purity by HPLC

 

• Size Exclusion HPLC
• Ion-Exchange HPLC  
• Reverse Phase HPLC 
• Peptide mapping 
• N-terminal sequencing
• CD-spectra analysis
• Bioactivity